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| Status |
Public on Mar 20, 2018 |
| Title |
Single-cell RNAseq analysis of the empty and i8TF cell lines after 3 days of BL-CFC culture |
| Organisms |
Homo sapiens; Mus musculus; other sequences |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The aim of the experiment was to assess the cell heterogeneity after the doxycycline treatment and the subsequent induction of the 8 transcription factors in the BL-CFC culture.
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| Overall design |
A2lox.empty and A2lox.i8TFs cell lines were used for this experiment. A2lox.empty is used a negative control while the doxycycline is inducing the expression of Erg, Fli1, Tal1, Lyl1, Lmo2, Runx1, Cbfb and Gata2 in the A2lox.i8TFs cell line. Cells were cultured in BL-CFC culture for 1 day before doxycycline treatment and were cultured for an additional 48hours with (i8TFs_plus and E_plus) or without doxycycline (i8TFs_minus and E_minus). As additional controls of the single cell RNA seq experiment, bulk A2lox.i8TFs cells were collected at the same time point from the condition without doxycycline (Pos_Ctrl), also buffer only was used as negative control (Neg_Ctrl) and a human cell line as other control (K562).
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| Contributor(s) |
Bergiers I, Lancrin C |
| Citation(s) |
29555020 |
| |
| Submission date |
Mar 23, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Christophe Lancrin |
| E-mail(s) |
christophe.lancrin@embl.it
|
| Organization name |
EMBL
|
| Department |
EMBL Rome
|
| Street address |
Via Ramarini 32
|
| City |
Monterotondo |
| State/province |
RM |
| ZIP/Postal code |
00015 |
| Country |
Italy |
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| Platforms (3) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24755 |
Illumina NextSeq 500 (other sequences) |
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| Samples (673)
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| This SubSeries is part of SuperSeries: |
| GSE96986 |
Single cell transcriptomics reveals new insights on the dynamical function of transcription factors during blood stem and progenitor cell formation |
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| Relations |
| BioProject |
PRJNA380304 |
| SRA |
SRP102429 |
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