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Status |
Public on Sep 07, 2017 |
Title |
ENAP1 is involved in ethylene response |
Organism |
Arabidopsis thaliana |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We report that ethylene regulate the ENAP1 binding. We perform a dual cross-linking procedure using the additional cross-linker ethylene glycol bis-succinimidylsuccinate (EGS; Thermo Scientific) along with formaldehyde according to (Pedmale et al. 2016) for Chip-sequencing (ChIPseq) of ENAP1, as well as ENAP1 ChIP- EIN2 reChIPseq (ChIP-reChIPseq), which were used chromatins isolated from 3-day old etiolated Col-0 seedlings treated with ethylene or air gas. Results show that ENAP1 is involved in the EIN2 dependent ethylene response.
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Overall design |
Examination of 2 different protein binding in 1 Arabidopsis lines which under 2 different treatment.
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Contributor(s) |
Qiao H, Wang L, Zhang F |
Citation(s) |
28874528 |
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Submission date |
Mar 31, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Hong Qiao |
Organization name |
The University of Texas at Austin
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Department |
Department of Molecular Biosciences
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Lab |
Qiao lab
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Street address |
2506 speedway, NMS5.324
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City |
Austin |
State/province |
TEXAS |
ZIP/Postal code |
78731 |
Country |
USA |
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Platforms (1) |
GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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Samples (6)
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Relations |
BioProject |
PRJNA381206 |
SRA |
SRP102817 |