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Series GSE97288 Query DataSets for GSE97288
Status Public on Sep 07, 2017
Title ENAP1 is involved in ethylene response
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report that ethylene regulate the ENAP1 binding. We perform a dual cross-linking procedure using the additional cross-linker ethylene glycol bis-succinimidylsuccinate (EGS; Thermo Scientific) along with formaldehyde according to (Pedmale et al. 2016) for Chip-sequencing (ChIPseq) of ENAP1, as well as ENAP1 ChIP- EIN2 reChIPseq (ChIP-reChIPseq), which were used chromatins isolated from 3-day old etiolated Col-0 seedlings treated with ethylene or air gas. Results show that ENAP1 is involved in the EIN2 dependent ethylene response.
Overall design Examination of 2 different protein binding in 1 Arabidopsis lines which under 2 different treatment.
Contributor(s) Qiao H, Wang L, Zhang F
Citation(s) 28874528
Submission date Mar 31, 2017
Last update date May 15, 2019
Contact name Hong Qiao
Organization name The University of Texas at Austin
Department Department of Molecular Biosciences
Lab Qiao lab
Street address 2506 speedway, NMS5.324
City Austin
State/province TEXAS
ZIP/Postal code 78731
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (6)
GSM2561047 COL_ENAP1_ChIPseq_air
GSM2561048 COL_ENAP1_ChIP_EIN2reChIP_air
GSM2561049 COL_ENAP1_air_input
BioProject PRJNA381206
SRA SRP102817

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97288_RAW.tar 910.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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