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| Status |
Public on Jul 12, 2017 |
| Title |
Yeast RNA-binding protein Nab3 regulations genes involved in nitrogen metabolism |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Termination of yeast RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while non-coding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected many non-coding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite repressed genes are up-regulated by Nab3 depletion.
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| |
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| Overall design |
Yeast Pol II genome wide positioning before and after Nab3-FRB tagged nuclear depletion using Illumina HI-Seq
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| |
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| Contributor(s) |
Merran JD, Corden JL |
| Citation missing |
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| Submission date |
Apr 03, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jonathan Merran |
| E-mail(s) |
jmerran1@jhu.edu
|
| Organization name |
Johns Hopkins University
|
| Department |
Molecular Biology and Genetics
|
| Lab |
Corden Lab
|
| Street address |
725 N. Wolfe Street
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21218 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA381477 |
| SRA |
SRP102940 |
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