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| Status |
Public on Aug 26, 2009 |
| Title |
Identification of differentially regulated genes in Populus deltoides subjected to copper stress using 4.6K cDNA arrays |
| Platform organism |
Populus trichocarpa x Populus deltoides |
| Sample organism |
Populus deltoides |
| Experiment type |
Expression profiling by array
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| Summary |
cDNA macroarray expression profiling was carried out in poplar roots in order to identify genes regulated in response to exposition to copper stress. For this purpose, plants of a Populus deltoides clone grown in a hydroponic system during four weeks were incubated in a nutrient solution (Hoagland's modiefied salt, ¼ strength) supplemented with copper (0 µM (control), 30 µM and 60 µM). Roots were sampled at 12 and 24 h after exposition in a time-course experiment.
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| Overall design |
Three biological replicates named Rep1, Rep2 and Rep3 were obtained for each copper treatment (i.e., control, Cu30 and Cu60) at each time point (i.e., 12, 24). At each time point, two comparisons were performed: control treatment versus Cu30 and control treatment versus Cu60. For each comparison, biological triplicates were used to identify transcripts differentially regulated at the considered timepoint using a statistical analysis approach based on linear mixed models by the SAS system.
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| Contributor(s) |
Guerra F, Duplessis S, Kohler A, Martin F, Tapia J, Lebed P, Zamudio F, González E |
| Citation |
Gene expression analysis of Populus deltoides roots subjected to copper stress. Environmental and Experimental Botany, 67(2): 335-344.
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| Submission date |
Dec 03, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Fernando Guerra |
| E-mail(s) |
fguerra@utalca.cl
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| Phone |
56-71-200281
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| Organization name |
Universidad de Talca
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| Department |
Instituto de Biologia Vegetal y Biotecnologia
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| Street address |
2 Norte 685
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| City |
Talca |
| State/province |
Talca |
| ZIP/Postal code |
0000000 |
| Country |
Chile |
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| Platforms (1) |
| GPL4887 |
Populus 4.6K cDNA macroarray |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA103671 |
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