|
| Status |
Public on Nov 13, 2017 |
| Title |
The Epstein-Barr virus episome maneuvers between nuclear chromatin compartments during reactivation [DNA-seq] |
| Organisms |
Homo sapiens; human gammaherpesvirus 4 |
| Experiment type |
Other
|
| Summary |
Using chromatin conformation capture methods, we learned that the latent episome of the human Epstein-Barr virus (EBV) displays preferential chromosome association that correlates with gene density. The episome avoids gene-rich chromosomes and favors gene-poor chromosomes. Kaposi’s sarcoma-associated herpesvirus behaves similarly, but human papillomavirus does not, suggesting limited evolutionary conservation of this strategy. Moreover, the strongest contacts we detected between the human genome and EBV episome localized to OriP, the latent origin of replication. This genetic element, and the EBNA1 protein that binds there, are sufficient to reconstitute chromosome association preferences of the entire episome. Upon reactivation from latency, however, these preferences are lost. Detailed mapping of changes in interchromosomal contacts reveal that the episome moves away from repressive heterochromatin and toward activating euchromatin. Our work adds three-dimensional relocalization to the molecular events that occur during the genetic switch from EBV latency to reactivation. The involvement of only a myriad of interchromosomal contacts also argues for a possible role of this type of long-range association in gene regulation.
|
| |
|
| Overall design |
DNA-seq examination of viral DNA in latent LGNFR- and reactivated LGNFR+ Akata-ZTA cells pre-treated with acyclovir and induced with doxycycline.
Please note that the wig files are a slightly modified format in that they display a viral genome not available as a chromosome on the UCSC genome browser. The wig files are readable by the IGV browser (as described in the associated publication).
|
| |
|
| Contributor(s) |
Moquin SA, Miranda JL |
| Citation(s) |
29142137 |
| |
| Submission date |
May 03, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
JJ Miranda |
| E-mail(s) |
jmiranda@barnard.edu
|
| Organization name |
Barnard College, Columbia University
|
| Street address |
3009 Broadway
|
| City |
New York |
| ZIP/Postal code |
10027 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23362 |
Illumina HiSeq 4000 (Homo sapiens; Human gammaherpesvirus 4) |
|
| Samples (10)
|
|
| This SubSeries is part of SuperSeries: |
| GSE98123 |
The Epstein-Barr virus episome maneuvers between nuclear chromatin compartments during reactivation |
|
| Relations |
| BioProject |
PRJNA385273 |
| SRA |
SRP106325 |
Less...
More...