|
Status |
Public on Dec 31, 2020 |
Title |
CD96+ and CD96- NK cells from human livers |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
To better define primary CD96+ NK cells and CD96- NK cells from human liver, we isolated primary hepatic lymphocytes from healthy liver and purified CD96+ and CD96- NK cells through negative selection and flow cytometry sorting. Purified NK cells from human liver tissues were first enriched by MACS using NK Cell Isolation Kit (Miltenyi Biotec, German) and CD96+/- hepatic NK cells were isolated by FACS Aria cell sorter (BD Biosciences, United States) to attain a purity greater than 95%.
|
|
|
Overall design |
Expression profiling of CD96+ hepatic NK and CD96- hepatic NK cell types. Three healthy donors were pooled for each cell type.
|
|
|
Contributor(s) |
Sun C |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
May 06, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Cheng Sun |
E-mail(s) |
charless@ustc.edu.cn
|
Organization name |
University of Science and Technology of China
|
Department |
the First Affiliated Hospital of USTC
|
Lab |
Department of Pathology
|
Street address |
17, Lujiang Road
|
City |
Hefei |
State/province |
Anhui |
ZIP/Postal code |
230036 |
Country |
China |
|
|
Platforms (1) |
GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
Samples (2) |
|
Relations |
BioProject |
PRJNA385693 |