GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE99399 Query DataSets for GSE99399
Status Public on Feb 23, 2018
Title A transcriptomic study of myogenic differentiation under the overexpression of PPARγ by RNA-Seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To study the cellular and molecular function of PPARγ in skeletal muscle differentiation, we have generated inducible gain-of-function to overexpress PPARγ in C2C12 skeletal muscle cells. In order to identify PPARγ targets, RNA sequencing (RNA-seq) was used to evaluate and quantify of transcriptomes and expression patterns during myogenic differentiation under the overexpression of PPARγ. The formation of myotubes and the expression of muscle-specific myogenic genes such as MyoD and MyoG may be inhibited by any altered expression of PPARγ. Multiple genes and pathways were significantly involved in this process, including 11 genes such as Fndc9 and Slc14a1 with fundamental change of regulation modes, 9 genes of which were validated by the data of qRT-PCR. Our studies demonstrate that PPARγ would play critical roles on the skeletal muscle cells differentiation, mediating crosstalk among several pathways and transcription factors.
Overall design Inducible PPARγ C2C12 cells were differentiated for 5 days. Uninduced cells were used as controls. Experiments were performed in triplicate. Gene expression profiling analyses by high throughput sequencing were performed on mRNA samples isolated from the sense PPARγ C2C12 cells (PPARγ/+) and the wildtype cells on 0d and 5d.
Contributor(s) He K, Wu G
Citation(s) 29127356
Submission date May 30, 2017
Last update date May 15, 2019
Contact name Kan He
Organization name Anhui University
Street address 111 Jiulong Road
City Hefei
State/province Anhui
ZIP/Postal code 230601
Country China
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM2643472 PbIP0d1(WT_0d_1)
GSM2643473 PbIP0d2(WT_0d_2)
GSM2643474 PbIP0d3(WT_0d_3)
BioProject PRJNA388391
SRA SRP108250

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99399_gene_sample_FPKM.xls.gz 2.2 Mb (ftp)(http) XLS
GSE99399_gene_sample_log2FPKM.xls.gz 2.2 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap