|
| Status |
Public on Mar 16, 2006 |
| Title |
DIV14 cortical neuron, KCl 1 |
| Sample type |
RNA |
| |
|
| Source name |
Rattus norvegicus embryonic day (E)17 cortex
|
| Organism |
Rattus norvegicus |
| Characteristics |
age: days of in vitro culture(DIV)14
|
| Treatment protocol |
Rat cortical neurons prepared on E17, cultured for 14 days were treated with 25mM KCl for 4hrs.
|
| Growth protocol |
cultured for 14days in Neurobasal media with B27 supplement at 37oC, 95% air, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNA extraction kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, MES buffer (2-[N-morpholino]ethansulfonic acid) containing 0.5mg biotinylated cRNA per 1ml actylated bovine serum albumin were hybridized for 16 hr at 45C onto RN U34 Genechip. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Description |
Gene expression data from na?ve DIV 14 cortical neuron i.e. non activated state
|
| Data processing |
The data were analyzed with Genechip Operating Software version 1.3 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 15, 2006 |
| Last update date |
Mar 15, 2006 |
| Contact name |
Hyun Kim |
| E-mail(s) |
kimhyun@korea.ac.kr
|
| Organization name |
College of Medicine, Korea university
|
| Street address |
Anam5ga
|
| City |
Seoul |
| ZIP/Postal code |
136-705 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL88 |
| Series (1) |
| GSE4472 |
Depolarization induced gene from primary cortical neuron |
|
