|
| Status |
Public on Jul 29, 2013 |
| Title |
brain_treated_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
25 month, rapamycin treated
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
strain: C57BL/6JRj
genotype: wildtype
age: 25 month
mouse id: 30210462-30
|
| Treatment protocol |
Male C57BL6/J Rj mice were obtained from Janvier (France) and were randomly assigned to rapamycin or vehicle treatment groups. We had three treatment cohorts, distinguished by age of treatment onset. Treatment was initiated in young adulthood (i.e., at approx. 4 months of age), in midlife (i.e., at 13 months of age) or late in life (i.e., at 20-22 months of age). Animals were treated for >12 months before analyses started at 16 months, 25 months and 34 months of age, respectively. Animals were kept on their respective diet for the entirety of the study. In all cases, the experimenter was blinded to the animal group assignments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using HiScan
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
|
| |
|
| Submission date |
Sep 20, 2012 |
| Last update date |
Jul 29, 2013 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
|
| Department |
Institute of Experimental Genetics
|
| Lab |
Gene Regulation & Epigenetics
|
| Street address |
Ingolstaedter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE41018 |
Rapamycin extends life span, but has limited effects on aging in mice |
|
