|
| Status |
Public on Jul 31, 2013 |
| Title |
Sox2 injections MZ CHX replicate_1 |
| Sample type |
RNA |
| |
|
| Source name |
Sox2 injections MZ CHX
|
| Organism |
Danio rerio |
| Characteristics |
genotype/variation: MZ mutant
tissue: whole embryo
microinjection: Sox2
|
| Treatment protocol |
Embryos of the genotypes indicated were injected with 100 pg/embryo Pou5f1 mRNA, 50 pg/embryo Sox2 mRNA or left uninjected. At 1.5 hpf, the embryos were placed into 15 mkg/ml Cycloheximide solution (Calbiochem), until the embryos were frozen at 4.7 hpf for RNA isolation
|
| Growth protocol |
WT (wild type) or MZ embryos carrying the m793 allele of the spg mutation were used
|
| Extracted molecule |
total RNA |
| Extraction protocol |
60-100 embryos per sample were snap-frozen in liquid nitrogen, and total RNA was isolated using the RNA Easy kit (Qiagen). Sample quality was assessed in an Agilent Bioanalyzer 2100, using the RNA 6000 nano Assay Kit
|
| Label |
Cy5
|
| Label protocol |
Samples were processed by Agilent Technologies Two-Color Microarray-Based Gene Expression Analysis kit according to Agilent Two-color Microarray based gene expression analysis protocoll
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit was used . Micorarrays were hybridized in the Agilent 2545A hybridization oven according Agilent Two-color Microarray based gene expression analysis protocoll
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner
|
| Description |
rebeecca_270508_251506410445_S01_GE2_107_Sep09_QC_1R
|
| Data processing |
Data were extracted using Agilent Feature Extraction Software and GE2-107_95_Sep09_QC protocoll.
Further Normalisation and processing of the data was done using Genedata Expressionist and Analyst software. Cy3 and Cy5 channels of each microarray were normalized using quantile normalization
|
| |
|
| Submission date |
Sep 24, 2012 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Daria Onichtchouk |
| E-mail(s) |
daria.onichtchouk@biologie.uni-freiburg.de
|
| Phone |
00497612032595
|
| Organization name |
University of Freiburg
|
| Street address |
Hauptstrasse 1
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7302 |
| Series (1) |
| GSE41107 |
Experiment 2: Pou5f1 and Sox2 overexpression experiments protein synthesis inhibition |
|
