|
| Status |
Public on Sep 26, 2012 |
| Title |
TYLCV infected LeHT1-silenced resistant line R Ri: TRV HEX 65974505 |
| Sample type |
RNA |
| |
|
| Source name |
TYLCV infected LeHT1-silenced resistance line
|
| Organism |
Solanum lycopersicum |
| Characteristics |
genotype: TYLCV resistant line R
infection: TYLCV infected
transfection: LeHT1 has been silenced using Tobacco rattle virus-induced gene silencing
tissue: whole plant
|
| Treatment protocol |
Five days after LeHT1 silencing, groups of twenty silenced and twenty non-silenced plants of the same age were inoculated with TYLCV by caging plants with viruliferous whiteflies for a 3-day long inoculation access period, (approximately 30 insects per plant), in insect-proof wooden cages kept at 24-27 ºC in an insect-proof growth chamber.
|
| Growth protocol |
Plants were grown in an insect-proof greenhouse kept at 18 ºC – 24 0C, with 8 h/16 h dark/light regimen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Reykjavik, Iceland, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc. Reykjavik, Iceland following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Reykjavik, Iceland, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Resistance plants where LeHT1 has been silenced using Tobacco rattle virus-induced gene silencing (TRV VIGS).
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Sep 25, 2012 |
| Last update date |
Sep 26, 2012 |
| Contact name |
Dagan Sade |
| Organization name |
Hebrew University of Jerusalem
|
| Street address |
Hertzel
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL16103 |
| Series (1) |
| GSE41135 |
Expression analysis of tomato plants TYLCV resistant, susceptible and resistant line silenced in the hexose transporter (LeHT1) gene before and 7 days after inoculation of tomato with Tomato yellow leaf curl virus (TYLCV). |
|
