|
| Status |
Public on Oct 10, 2012 |
| Title |
Non-isoflavone diet, biological rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Non-isoflavone diet (control)
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
gender: male
tissue: liver
age: Experiment was started at 4 wks of age, rats were adapted to the condition for 5 days and fed the diet indicated for 14 days.
diet: control (Non-isoflavone)
|
| Treatment protocol |
Three groups of rats were fed an experimental diet containing 2 g/kg of either genistein or daidzein, or a control diet free of isoflavone for 14 days. The basal composition of the experimental diet was (in g/kg): casein, 200; palm oil, 100; corn starch, 150; cellulose, 20; mineral mixture (AIN-93G), 35; vitamin mixture (AIN-93), 10; L-cystine, 3.0; choline bitartrate, 2.0 and sucrose to 1 kg.
|
| Growth protocol |
Male Sprague Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 weeks of age. The animals were housed individually in a room with controlled temperature and humidity and a 12 h light dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
One-Cycle Target Labeling and Control Reagents (Affymetrix)
|
| |
|
| Hybridization protocol |
GeneChip® Hybridization Oven 640 (Affymetrix)
|
| Scan protocol |
GeneChip® Scanner 3000 (Affymetrix）
|
| Description |
Control 8
|
| Data processing |
The perfect match data were normalized by using the SuperNORM data service (Skylight Biotech Inc., Akita, Japan) according to a three-parameter lognormal distribution model [details on PMID 18764774, 18958174, 22784577]. The log values are log10 (rawdata - gamma): z-score = (log10 (rawdata- gamma) - mu)/sigma.
|
| |
|
| Submission date |
Oct 09, 2012 |
| Last update date |
Oct 10, 2012 |
| Contact name |
Tomokazu Konishi |
| E-mail(s) |
konishi@akita-pu.ac.jp
|
| Phone |
+81-18-872-1603
|
| Organization name |
Akita Prefectural University
|
| Department |
Bioresource Sciences
|
| Lab |
Molecular Genetics
|
| Street address |
Shimoshinjyo Nishi
|
| City |
Akita |
| State/province |
Akita |
| ZIP/Postal code |
010-0195 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE41420 |
A comparative analysis of genistein and daidzein in affecting lipid metabolism in rat liver |
|
