|
| Status |
Public on Nov 20, 2013 |
| Title |
Nr5_ler |
| Sample type |
RNA |
| |
|
| Source name |
ler; replicate 1
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: ler
|
| Growth protocol |
Arapidopsis thaliana plants were grown in soil and cultivated in a greenhouse under short-day conditions (8 h light/16 h darkness, 22°C/16°C) with illumination by 100 µmol m-2 sec-1 white light. Samples were collected after 5h of illumination.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plant Mini Kit, according to the manufacturers protocol
|
| Label |
Cyanin3
|
| Label protocol |
One-color microarray-based gene expression analysis protocol provided by Agilent including the one-color RNA spike-in kit (v5.0.1, 2006)
|
| |
|
| Hybridization protocol |
Samples were hybridized on an Agilent Platform using the Agilent Arabidopsis V4 (design number 021169) microarray chips (http://www.agilent.com) with one-color microarray-based gene expression analysis protocol provided by Agilent including the one-color RNA spike-in kit (v5.0.1, 2006).
|
| Scan protocol |
Agilent Microarray Scanner,extended dynamic range (XDR) at high resolution (5 µm)
|
| Description |
enriched guard cell fraction of Arabidopsis thaliana
|
| Data processing |
Data sets were extracted by the feature extraction software package (v9.5.3.1/ Agilent Technologies) using a standard protocol. Subsequently data were normalized using quantile-quantile as normalization method (bioconductor package "preprocessCore").
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Nov 20, 2013 |
| Contact name |
Susanne Elma Kneitz |
| E-mail(s) |
susanne.kneitz@uni-wuerzburg.de
|
| Phone |
+49-931-31 86526
|
| Organization name |
University of Wuerzburg
|
| Department |
Physiologigcal Chemistry
|
| Street address |
Biozentrum, Am Hubland
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9020 |
| Series (1) |
| GSE42470 |
Agilent data from Arabidopsis thaliana enriched guard cell tissue of ost1-2,slac 1-5, gl1-1 and landsberg erecta |
|
