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Sample GSM1048762 Query DataSets for GSM1048762
Status Public on Dec 05, 2012
Title BMDC_Control
Sample type RNA
Source name BMDC_control
Organism Mus musculus
Characteristics cell type: Bone Marrow-derived Dendritic Cells (BMDCs)
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated using the TRIZOL® Reagent(Invitrogen) according to manufacturer’s instructions.
Label Hy3
Label protocol The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Cat #208032-A, Exiqon) according to manufacturer's instructions.
Hybridization protocol The labeled samples were hybridized on the miRCURY LNA™ microRNA Array V11.0.
Scan protocol Hybridization signals were detected by fluorescence using an GenePix 4000B scanner (Molecular Devices). Scanner images were quantified by GenePix Pro 6 (Molecular Devices).
Description RY2009_01
Data processing For each spot, verify the signal by subtracting background intensity (B) from foreground intensity (F). Verified signal value= F - B. The spots, of which verified signal values are less than 50, and the control spots are filtered. For verified signal values of the left spots, perform median normalization.
Submission date Dec 04, 2012
Last update date Dec 05, 2012
Contact name Yang Rongcun
Phone +86-(22)-23509007
Fax +86-(22)-23502554
Organization name Nankai University
Department School of medicine
Street address 94 Weijin Road, Nankai District
City Tianjin, P.R.China
ZIP/Postal code 300071
Country China
Platform ID GPL16352
Series (1)
GSE42722 miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
1001 14.48538462
1002 -0.003846154
1003 0.136153846
1004 -0.002307692
1005 -0.010769231
1006 0.134615385
1007 0.016923077
1008 0.379230769
1009 -0.007692308
1010 0
1011 0.060769231
1012 -0.001538462
1013 0.006153846
1014 0.201538462
1015 0.020769231
1016 14.79615385
1017 0.012307692
1018 0.067692308
1019 0.025384615
1020 0.001538462

Total number of rows: 1408

Table truncated, full table size 22 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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