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Sample GSM1048764 Query DataSets for GSM1048764
Status Public on Dec 05, 2012
Title BMDC_CT-26
Sample type RNA
Source name BMDC_tumor cells
Organism Mus musculus
Characteristics cell type: Bone Marrow-derived Dendritic Cells (BMDCs)
co-cultured with: CT-26 tumor cells
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated using the TRIZOL® Reagent(Invitrogen) according to manufacturer’s instructions.
Label Hy3
Label protocol The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Cat #208032-A, Exiqon) according to manufacturer's instructions.
Hybridization protocol The labeled samples were hybridized on the miRCURY LNA™ microRNA Array V11.0.
Scan protocol Hybridization signals were detected by fluorescence using an GenePix 4000B scanner (Molecular Devices). Scanner images were quantified by GenePix Pro 6 (Molecular Devices).
Description RY2009_03
Data processing For each spot, verify the signal by subtracting background intensity (B) from foreground intensity (F). Verified signal value= F - B. The spots, of which verified signal values are less than 50, and the control spots are filtered. For verified signal values of the left spots, perform median normalization.
Submission date Dec 04, 2012
Last update date Dec 05, 2012
Contact name Yang Rongcun
Phone +86-(22)-23509007
Fax +86-(22)-23502554
Organization name Nankai University
Department School of medicine
Street address 94 Weijin Road, Nankai District
City Tianjin, P.R.China
ZIP/Postal code 300071
Country China
Platform ID GPL16352
Series (1)
GSE42722 miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
1001 13.18344519
1002 0.008948546
1003 0.117076808
1004 -0.002237136
1005 -0.004474273
1006 0.178225205
1007 -0.004474273
1008 0.3251305
1009 0.009694258
1010 -0.004474273
1011 0.045488441
1012 0.000745712
1013 0.012677107
1014 0.818791946
1015 0.006711409
1016 12.59806115
1017 0.000745712
1018 0.023117077
1019 0.01715138
1020 0.012677107

Total number of rows: 1408

Table truncated, full table size 23 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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