|
Status |
Public on Dec 05, 2012 |
Title |
BMDC_CT-26 |
Sample type |
RNA |
|
|
Source name |
BMDC_tumor cells
|
Organism |
Mus musculus |
Characteristics |
cell type: Bone Marrow-derived Dendritic Cells (BMDCs) co-cultured with: CT-26 tumor cells
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were isolated using the TRIZOL® Reagent(Invitrogen) according to manufacturer’s instructions.
|
Label |
Hy3
|
Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Cat #208032-A, Exiqon) according to manufacturer's instructions.
|
|
|
Hybridization protocol |
The labeled samples were hybridized on the miRCURY LNA™ microRNA Array V11.0.
|
Scan protocol |
Hybridization signals were detected by fluorescence using an GenePix 4000B scanner (Molecular Devices). Scanner images were quantified by GenePix Pro 6 (Molecular Devices).
|
Description |
RY2009_03
|
Data processing |
For each spot, verify the signal by subtracting background intensity (B) from foreground intensity (F). Verified signal value= F - B. The spots, of which verified signal values are less than 50, and the control spots are filtered. For verified signal values of the left spots, perform median normalization.
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|
|
Submission date |
Dec 04, 2012 |
Last update date |
Dec 05, 2012 |
Contact name |
Yang Rongcun |
E-mail(s) |
ryang@nankai.edu.cn
|
Phone |
+86-(22)-23509007
|
Fax |
+86-(22)-23502554
|
Organization name |
Nankai University
|
Department |
School of medicine
|
Street address |
94 Weijin Road, Nankai District
|
City |
Tianjin, P.R.China |
ZIP/Postal code |
300071 |
Country |
China |
|
|
Platform ID |
GPL16352 |
Series (1) |
GSE42722 |
miRNA expression profiling in tumor-associated BMDCs induced by mouse ovarian and colon tumor cells |
|