|
| Status |
Public on Aug 15, 2013 |
| Title |
mosaic larval eye-antennal discs RasACT overexpressing scrib mutant 2 |
| Sample type |
RNA |
| |
|
| Source name |
RasV12 expressing scrib mutant Drosophila larval mosaic eye-antennal discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: larval mosaic eye-antennal discs
genotype: RasV12 expressing scrib mutant
developmental stage: 3rd instar larvae
genetic background: w1118
|
| Treatment protocol |
eye-annennal disc were dissected from 3rd instar larvae at day 5 after egg deposition.
|
| Growth protocol |
Larvae were incubated at 25oC and grown on standard fly media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were prepared in triplicate, and the RNA isolated using TRIZOL, before being column purified (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Hybridization was carried out following standard Affymetrix protocols
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 following Affymetrix protocols.
|
| Description |
tumours
|
| Data processing |
The data was gcrma normalised using the R Bioconductor package gcrma.
|
| |
|
| Submission date |
Dec 14, 2012 |
| Last update date |
Aug 15, 2013 |
| Contact name |
Helena Elizabeth Richardson |
| E-mail(s) |
helena.richardson@petermac.org
|
| Phone |
61396561466
|
| Organization name |
Peter MacCallum Cancer Center
|
| Department |
Research Division
|
| Lab |
Cell Cycle and Development lab
|
| Street address |
7 St Andrew's place
|
| City |
East Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3002 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE42938 |
Abrupt scrib- vs. Abrupt and RasV12 (RasACT) scrib-, NotchICD (NACT) scrib- +/- JNK (Bsk) expression profiles |
|
