|
Status |
Public on Apr 01, 2013 |
Title |
cartilage knee 10SE213 |
Sample type |
RNA |
|
|
Source name |
cartilage knee
|
Organism |
Homo sapiens |
Characteristics |
disease state: osteoarthritis age: 79 Sex: Female haplogroup: T tissue: knee cartilage
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from crushed tissue using the Rneasy Mini kit (Qiagen) following manufacturer instructions, with the addition of a DNAse digestion step.
|
Label |
biotin
|
Label protocol |
Fragmented cDNA was labelled using the WT Terminal Labelling kit (Affymetrix)
|
|
|
Hybridization protocol |
Washing and staining using kit for WT array plates, following Affymetrix protocol, Hybridization was carried out using GeneTitan Hybridization
|
Scan protocol |
Scan was performed on GeneTitan instrument
|
Data processing |
CEL files were read with oligo package and subsequently analyzed with the same package. RMA algorithm was applied and a filter step based on annotation was also included in the analysis. pd.hugene.1.1.st.v1 hugene11sttranscriptcluster.db
|
|
|
Submission date |
Dec 28, 2012 |
Last update date |
Apr 01, 2013 |
Contact name |
Juan Fernandez Tajes |
E-mail(s) |
jfernandezt@udc.es
|
Organization name |
INIBIC
|
Department |
Genomics Area
|
Street address |
As Xubias
|
City |
A Coruña |
ZIP/Postal code |
15006 |
Country |
Spain |
|
|
Platform ID |
GPL11532 |
Series (2) |
GSE43191 |
Genome wide-DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients (gene expression) |
GSE43270 |
Genome wide-DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients |
|