|
| Status |
Public on Jul 02, 2013 |
| Title |
B-cell lymphoma, Dog 8 |
| Sample type |
RNA |
| |
|
| Source name |
Lymph node
|
| Organism |
Canis lupus familiaris |
| Characteristics |
vet path diagnosis: DLBCL
ighv status: ongoing
pfs time: 285
pfs indicator: 1
OS time: 493
os indicator: 1
|
| Treatment protocol |
Biopsies were obtained prior to treatment and B-cell lymphomas were identified by flow cytometry and/or immunohistochemistry for B-cell markers
|
| Growth protocol |
Client owned pet dogs presented for veterinary care
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
One microgram of total RNA was processed for microarray hybridization to Affymetrix GeneChips using the MessageAmp II-Biotin Enhanced Kit from Ambion according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation at 94°C for 35 min, the aRNA was prepared for hybridization according to the Affymetrix Expession Analysis Technical Manual. The hybridization cocktail was hybridized to the arrays at 45°C for 16 hours. The arrays were washed with the Affymetrix Fluidics Station 450 wash stations.
|
| Scan protocol |
The arrays were scanned with the Affymetrix GeneChip Scanner 3000 7G Plus with Autoloader.
|
| Description |
Gene expression data from canine B-cell lymphoma patient
|
| Data processing |
Basic data analysis and quality of the arrays was carried out using Affymetrix Expression Console software
Microarray data were normalized using RMA implemented by the Bioconductor package in R10, version 2.12.1.
|
| |
|
| Submission date |
Jan 22, 2013 |
| Last update date |
May 04, 2016 |
| Contact name |
Kristy L Richards |
| E-mail(s) |
kristy_richards@med.unc.edu
|
| Phone |
919-966-0374
|
| Organization name |
University of North Carolina
|
| Department |
Medicine
|
| Street address |
1062 Genetic Medicine Bldg.
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7361 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE43664 |
Expression data from canine B-cell lymphomas |
|
| Relations |
| Reanalyzed by |
GSE81110 |
