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Sample GSM1068489 Query DataSets for GSM1068489
Status Public on Jul 01, 2014
Title Bronchial Epithelial Cells MMA biological 2119J
Sample type RNA
 
Source name Human Fresh Bronchial Epithelial cells
Organism Homo sapiens
Characteristics disease state: Moderate Asthma
cell type: Fresh Bronchial Epithelial Cells
gender: male
age: 21.45479
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-CTP labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
 
Hybridization protocol Labeled RNA was hybridized for 17 hr at 65C on Agilent gene expression array. Arrays were washed according to the manufacturer's protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
Description Freshly isolated Bronchial Epithelial Cells
Bronchoscopy with endobronchial epithelial brushing was performed as previously described (Chu et al., 2002; Zhao et al., 2011). The bronchial brushings generally comprised >90% epithelial cells and were placed into Trizol for mRNA analysis.
Data processing Agilent Feature Extraction v10.7.3.1 default parameters
 
Submission date Jan 23, 2013
Last update date Jul 02, 2014
Contact name Jadranka Milosevic
E-mail(s) jam206@pitt.edu
Organization name University of Pittsburgh
Street address 2330 Tilbury Avenue
City Pittsburgh
State/province PA - Pennsylvania
ZIP/Postal code 15217
Country USA
 
Platform ID GPL6480
Series (1)
GSE43696 Asthma

Data table header descriptions
ID_REF
VALUE log2 Agilent Feature Extraction default normalized signal intensity

Data table
ID_REF VALUE
A_24_P66027 5.895495442
A_32_P77178 2.924597271
A_23_P212522 7.787980609
A_24_P934473 4.446186709
A_24_P9671 12.2382011
A_32_P29551 3.841092418
A_24_P801451 6.15661524
A_32_P30710 14.4261299
A_32_P89523 2.739461326
A_24_P704878 1.421058356
A_32_P86028 14.66426241
A_24_P470079 7.484337157
A_23_P65830 9.802743391
A_24_P595567 7.902762122
A_24_P391591 9.947578669
A_24_P799245 1.376949095
A_24_P932757 1.291234414
A_24_P835500 10.58447399
A_23_P54340 1.285981875
A_23_P67555 5.964101859

Total number of rows: 41000

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM1068489_2119J.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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