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Sample GSM1068497 Query DataSets for GSM1068497
Status Public on Jul 01, 2014
Title Bronchial Epithelial Cells MMA biological 1765J
Sample type RNA
 
Source name Human Fresh Bronchial Epithelial cells
Organism Homo sapiens
Characteristics disease state: Moderate Asthma
cell type: Fresh Bronchial Epithelial Cells
gender: male
age: 29.26301
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-CTP labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
 
Hybridization protocol Labeled RNA was hybridized for 17 hr at 65C on Agilent gene expression array. Arrays were washed according to the manufacturer's protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
Description Freshly isolated Bronchial Epithelial Cells
Bronchoscopy with endobronchial epithelial brushing was performed as previously described (Chu et al., 2002; Zhao et al., 2011). The bronchial brushings generally comprised >90% epithelial cells and were placed into Trizol for mRNA analysis.
Data processing Agilent Feature Extraction v10.7.3.1 default parameters
 
Submission date Jan 23, 2013
Last update date Jul 02, 2014
Contact name Jadranka Milosevic
E-mail(s) jam206@pitt.edu
Organization name University of Pittsburgh
Street address 2330 Tilbury Avenue
City Pittsburgh
State/province PA - Pennsylvania
ZIP/Postal code 15217
Country USA
 
Platform ID GPL6480
Series (1)
GSE43696 Asthma

Data table header descriptions
ID_REF
VALUE log2 Agilent Feature Extraction default normalized signal intensity

Data table
ID_REF VALUE
A_24_P66027 3.896602161
A_32_P77178 1.507623979
A_23_P212522 7.522966951
A_24_P934473 4.472337212
A_24_P9671 11.89189866
A_32_P29551 1.715977488
A_24_P801451 6.232786693
A_32_P30710 14.72572705
A_32_P89523 3.609224828
A_24_P704878 1.858178244
A_32_P86028 15.0607188
A_24_P470079 6.653864312
A_23_P65830 10.32265559
A_24_P595567 8.08943608
A_24_P391591 7.743651427
A_24_P799245 1.524670526
A_24_P932757 1.525027505
A_24_P835500 10.5893332
A_23_P54340 1.602930837
A_23_P67555 6.09643741

Total number of rows: 41000

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM1068497_1765J.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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