|
Status |
Public on Jul 01, 2014 |
Title |
Bronchial Epithelial Cells SA biological 0658D |
Sample type |
RNA |
|
|
Source name |
Human Fresh Bronchial Epithelial cells
|
Organism |
Homo sapiens |
Characteristics |
disease state: Severe Asthma cell type: Fresh Bronchial Epithelial Cells gender: female age: 43.59178
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Cy3-CTP labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
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|
|
Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent gene expression array. Arrays were washed according to the manufacturer's protocol.
|
Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
|
Description |
Freshly isolated Bronchial Epithelial Cells Bronchoscopy with endobronchial epithelial brushing was performed as previously described (Chu et al., 2002; Zhao et al., 2011). The bronchial brushings generally comprised >90% epithelial cells and were placed into Trizol for mRNA analysis.
|
Data processing |
Agilent Feature Extraction v10.7.3.1 default parameters
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|
Submission date |
Jan 23, 2013 |
Last update date |
Jul 02, 2014 |
Contact name |
Jadranka Milosevic |
E-mail(s) |
jam206@pitt.edu
|
Organization name |
University of Pittsburgh
|
Street address |
2330 Tilbury Avenue
|
City |
Pittsburgh |
State/province |
PA - Pennsylvania |
ZIP/Postal code |
15217 |
Country |
USA |
|
|
Platform ID |
GPL6480 |
Series (1) |
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