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Sample GSM1068528 Query DataSets for GSM1068528
Status Public on Jul 01, 2014
Title Bronchial Epithelial Cells SA biological 0658D
Sample type RNA
 
Source name Human Fresh Bronchial Epithelial cells
Organism Homo sapiens
Characteristics disease state: Severe Asthma
cell type: Fresh Bronchial Epithelial Cells
gender: female
age: 43.59178
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-CTP labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
 
Hybridization protocol Labeled RNA was hybridized for 17 hr at 65C on Agilent gene expression array. Arrays were washed according to the manufacturer's protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
Description Freshly isolated Bronchial Epithelial Cells
Bronchoscopy with endobronchial epithelial brushing was performed as previously described (Chu et al., 2002; Zhao et al., 2011). The bronchial brushings generally comprised >90% epithelial cells and were placed into Trizol for mRNA analysis.
Data processing Agilent Feature Extraction v10.7.3.1 default parameters
 
Submission date Jan 23, 2013
Last update date Jul 02, 2014
Contact name Jadranka Milosevic
E-mail(s) jam206@pitt.edu
Organization name University of Pittsburgh
Street address 2330 Tilbury Avenue
City Pittsburgh
State/province PA - Pennsylvania
ZIP/Postal code 15217
Country USA
 
Platform ID GPL6480
Series (1)
GSE43696 Asthma

Data table header descriptions
ID_REF
VALUE log2 Agilent Feature Extraction default normalized signal intensity

Data table
ID_REF VALUE
A_24_P66027 6.028744065
A_32_P77178 2.336782331
A_23_P212522 7.674244908
A_24_P934473 4.772674761
A_24_P9671 11.74948169
A_32_P29551 2.477159016
A_24_P801451 5.366952149
A_32_P30710 15.02167506
A_32_P89523 4.686098588
A_24_P704878 1.964482282
A_32_P86028 15.54126251
A_24_P470079 6.31063863
A_23_P65830 10.02191723
A_24_P595567 8.195198563
A_24_P391591 8.665205361
A_24_P799245 1.613220683
A_24_P932757 1.614122751
A_24_P835500 11.24018297
A_23_P54340 2.037292168
A_23_P67555 6.477066109

Total number of rows: 41000

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM1068528_0658D.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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