|
| Status |
Public on Jul 01, 2014 |
| Title |
Bronchial Epithelial Cells SA biological 2118H |
| Sample type |
RNA |
| |
|
| Source name |
Human Fresh Bronchial Epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Severe Asthma
cell type: Fresh Bronchial Epithelial Cells
gender: male
age: 46.68219
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3-CTP labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent gene expression array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
|
| Description |
Freshly isolated Bronchial Epithelial Cells
Bronchoscopy with endobronchial epithelial brushing was performed as previously described (Chu et al., 2002; Zhao et al., 2011). The bronchial brushings generally comprised >90% epithelial cells and were placed into Trizol for mRNA analysis.
|
| Data processing |
Agilent Feature Extraction v10.7.3.1 default parameters
|
| |
|
| Submission date |
Jan 23, 2013 |
| Last update date |
Jul 02, 2014 |
| Contact name |
Jadranka Milosevic |
| E-mail(s) |
jam206@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Street address |
2330 Tilbury Avenue
|
| City |
Pittsburgh |
| State/province |
PA - Pennsylvania |
| ZIP/Postal code |
15217 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
|
