|
| Status |
Public on Jan 31, 2013 |
| Title |
iPS-NHDF-2 |
| Sample type |
RNA |
| |
|
| Source name |
adult normal human dermal fibroblast-derived induced pluripotent stem cell line
|
| Organism |
Homo sapiens |
| Characteristics |
culture medium: mTeSR-1 + matrigel
|
| Treatment protocol |
untreated cell samples, all grown in mTeSR-1 medium + ES-qualified matrigel prior to harvest
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit and with on-column Dnase treatment. Quality control was performed with Agilent Bioanalyser and was RIN 10 for each sample.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared as standard for Illumina arrays, by Wellcome Trust Centre for Human Genomics, Oxford
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 3
|
| Data processing |
Quality control results were within expected range. The data were normalised using cubic spline normalisation with background subtraction in GenomeStudio v2011.1 Gene Expression module 1.9.0. Missing values were imputed by the software. Manifest file used was HumanHT-12_V4_R2_15002873_B
|
| |
|
| Submission date |
Jan 30, 2013 |
| Last update date |
Jan 31, 2013 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
sally.cowley@path.ox.ac.uk
|
| Phone |
+44 (0) 1865 275600
|
| Organization name |
University of Oxford
|
| Department |
Sir William Dunn School of Pathology
|
| Lab |
James Martin Stem Cell Facility
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE43903 |
Gene expression data from human induced Pluripotent Stem cells |
| GSE43904 |
SNP and expression data from human induced Pluripotent Stem cells derived from normal human dermal fibroblasts |
|
