|
| Status |
Public on Jan 31, 2013 |
| Title |
WT_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
E12.5 whole embryo
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: embryonic day E12.5
tissue: whole embryo
genotype: Klf3+/+
|
| Treatment protocol |
RNA was extracted from E12.5 embryos resulting from timed matings
|
| Growth protocol |
All mice were housed and maintained in standard facilities in accordance with the guidelines of the Canadian Council of Animal Care.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using TRI-Reagent, according to manufacturer’s guidelines (Sigma-Aldrich). RNA samples were further purified using RNeasy columns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the Affymetrix IVT Express kit
|
| |
|
| Hybridization protocol |
RNA was hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays following manufacturers instructions, by the Ramaciotti Centre for Gene Function, UNSW, Sydney, Australia
|
| Scan protocol |
Arrays were scanned following manufacturers instructions, by the Ramaciotti Centre for Gene Function, UNSW, Sydney, Australia
|
| Data processing |
Microarray data were analyzed using Partek Genomic Suite v6.6 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
|
| |
|
| Submission date |
Jan 30, 2013 |
| Last update date |
Jan 31, 2013 |
| Contact name |
Alister Funnell |
| E-mail(s) |
a.funnell@unsw.edu.au
|
| Organization name |
University of NSW
|
| Street address |
School of Biotechnology and Biomolecular Sciences
|
| City |
University of NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE43908 |
Microarray analysis of wildtype, Klf3 H275R homozygous and heterozygous, Klf3 CH gene trap homozygous and heterozygous E12.5 embryos |
|
