|
| Status |
Public on Dec 22, 2006 |
| Title |
6microg/L_Cu_Exposed_rep2B |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
whole organism, exposed 24-h to 6 microg/L Cu
|
| Organism |
Daphnia magna |
| Characteristics |
Daphnia magna: Aquatic Research Organisms
Age: 15-18 days
|
| Biomaterial provider |
Vulpe Lab
|
| Treatment protocol |
24-h exposure to 6 microg/L Copper Sulfate
|
| Growth protocol |
Genetically homogeneous Daphnia magna, purchased from Aquatic Research Organisms (Hampton, NH), were cultured in COMBO modified for water hardness (Kilham et al. 1998) and kept at 23.50 C in a Percival environmental chamber with a 16-hour light/ 8 hour dark cycle. The D. magna were maintained on a diet of YCT and Selenastrum capricornatum (Aquatic Research Organisms, Hampton, NH). Stock culture medium was renewed, cultures were fed, and neonates were separated from adults three times weekly.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method (invitrogen)
|
| Label |
cy3
|
| Label protocol |
see the protocols at the website of Dr. Patrick O. Brown's laboratory at Stanford University
|
| |
|
| Channel 2 |
| Source name |
whole organism, unexposed
|
| Organism |
Daphnia magna |
| Characteristics |
untreated 24 hours
|
| Biomaterial provider |
Vulpe Lab
|
| Treatment protocol |
24-h exposure to 6 microg/L Copper Sulfate
|
| Growth protocol |
Genetically homogeneous Daphnia magna, purchased from Aquatic Research Organisms (Hampton, NH), were cultured in COMBO modified for water hardness (Kilham et al. 1998) and kept at 23.50 C in a Percival environmental chamber with a 16-hour light/ 8 hour dark cycle. The D. magna were maintained on a diet of YCT and Selenastrum capricornatum (Aquatic Research Organisms, Hampton, NH). Stock culture medium was renewed, cultures were fed, and neonates were separated from adults three times weekly.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol method (Invitrogen)
|
| Label |
cy5
|
| Label protocol |
see the protocols at the website of Dr. Patrick O. Brown's laboratory at Stanford University
|
| |
|
| |
| Hybridization protocol |
see the protocols at the website of Dr. Patrick O. Brown's laboratory at Stanford University
|
| Scan protocol |
Scanning was performed using an arrayWoRx Biochip Reader (Applied Precision, Issaquah, WA)
|
| Description |
none
|
| Data processing |
Signal intensities determined by GenePix software version 3.01 (Axon Instruments, Union City, CA)
|
| |
|
| Submission date |
May 02, 2006 |
| Last update date |
Dec 22, 2006 |
| Contact name |
Helen C Poynton |
| E-mail(s) |
helen.poynton@umb.edu
|
| Phone |
617-287-7323
|
| Organization name |
UMass Boston
|
| Department |
School for the Environment
|
| Lab |
Poynton Lab
|
| Street address |
100 Morrissey Blvd.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platform ID |
GPL3710 |
| Series (1) |
| GSE4759 |
1/10 LC50 metal exposures to Daphnia magna |
|
