|
| Status |
Public on Jul 31, 2006 |
| Title |
Daudi_AICAR1h_antiCD40+IL4_6h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Daudi cells
|
| Organism |
Homo sapiens |
| Characteristics |
Burkitt's lymphoma
16-year-old Black male
|
| Treatment protocol |
Daudi cells were treated with 2.5 mM AICAR hor 1 hr, then stimulated with 1 ug/mL anti-CD40 and 10 ng/mL IL-4 for 6 hr. After wash with cold PBS twice, cells were lysed in RLT buffer. Total RNA was extracted using an RNeasy
|
| Growth protocol |
Daudi cells were cutured in RPMI1640 with 10% FBS. Cells were treated with 2.5 mM AICAR for 1 hr, then stimulated with agonistic anti-CD40 and IL-4 for 6 hr.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with CodeLink Expression Assay Kit according to the standard manufacture's protocol from 2 microg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 18 hr at 37C on CodeLink Human Whole Genome Bioarrays. Bioarrayss were washed and stained with Cy-5 Streptavidin
|
| Scan protocol |
Bioarrays were scanned using arrayWoRx scanner.
|
| Description |
none
|
| Data processing |
CodeLink Expression Analysis software was used for primary data (raw data) extraction from bioarray images. Multiple bioarray data were normalized by mean using Spotfire.
|
| |
|
| Submission date |
May 11, 2006 |
| Last update date |
May 11, 2006 |
| Contact name |
Hiroshi Fukasawa |
| E-mail(s) |
fukasawa@immd.co.jp
|
| Organization name |
Institute of Medicinal Molecular Design, Inc.
|
| Street address |
Kadokawa Hongo Bldg. 4F, 5-24-5 Hongo
|
| City |
Bunkyo-ku, Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE4812 |
Expression data from Daudi cells stimulated with anti-CD40 and/or IL-4 for 6 hr with or without AICAR-pretreatment |
|
