|
| Status |
Public on Mar 14, 2013 |
| Title |
Trp63 cHet uterus, rep4 |
| Sample type |
RNA |
| |
|
| Source name |
cHet-UT4
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: mixed (C57BL/6, S129/5 and CD-1)
genotype: Trp63 flox/wt ; Pax2-cre
tissue: uterus
gender: female
age: postnatal day 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Total RNA was extracted from the uteri of 3 Trp63 cHet mice.
|
| Data processing |
The average signal intensity for each array was normalized to the average total signal intensity of all 12 arrays by average normalization utilizing Excel.
|
| |
|
| Submission date |
Mar 13, 2013 |
| Last update date |
Mar 14, 2013 |
| Contact name |
Takeshi Kurita |
| E-mail(s) |
takeshi.kurita@osumc.edu
|
| Phone |
312-503-0525
|
| Organization name |
Ohio State University
|
| Department |
Molecular Virology, Immunology & Medical Genetics
|
| Street address |
460 W. 12th Avenue,
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE44697 |
Effect of Trp63 deletion on the developing vagina |
|
