|
| Status |
Public on Mar 21, 2013 |
| Title |
P53-wildetype without Del17p, biological rep64 |
| Sample type |
RNA |
| |
|
| Source name |
Patient WITHOUT P53-mutation and WITHOUT Del17p
|
| Organism |
Homo sapiens |
| Characteristics |
p53 mutation: NO
del17p: NO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of CD19 selected B-cells was isolated using Ambion miRVANA total RNA extraction kit
|
| Label |
biotin
|
| Label protocol |
The 3’ ends of RNA molecules in total RNA samples (400ng total RNA per sample) were labeled with biotin according to the company’s standard protocol
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Affymetrix-recommended procedures, except that the 20X GeneChip Eukaryotic Hybridization Controls were omitted from the hybridization.
|
| Scan protocol |
Imaging and signal extraction were performed according to Affymetrix-recommended procedures. GCOS 1.3 was used
|
| Data processing |
The probe intensities of miRNA arrays were background-subtracted, quantile normalized and summarized using the RMA method. All data were log2-transformed
|
| |
|
| Submission date |
Mar 20, 2013 |
| Last update date |
Mar 21, 2013 |
| Contact name |
Giuseppe Palermo |
| E-mail(s) |
giuseppe.palermo@roche.com
|
| Organization name |
F. Hoffman-La Roche Ltd.
|
| Street address |
wettsteinallee 76
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL8733 |
| Series (1) |
| GSE45328 |
Inactivation of TP53 correlates with disease progression and low miR-34a expression in previously treated chronic lymphocytic leukemia patients |
|
