|
| Status |
Public on Jul 14, 2014 |
| Title |
3 treatment using 0.08ug, 0.8ug, 8ug peptide prime |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells isolated from mice
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Tg4 Rag1 deficient TCR-transgenic mice
cell type: CD4+ T
treatment: 0.08ug, 0.8ug, 8ug peptide prime
|
| Treatment protocol |
CD4+ T cells isolated from mice at six stages of dose escalation immunotherapy or a PBS-treated control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy (Qiagen) column-based extraction of total RNA, with DNase digestion was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The fragmented, biotinylated cRNA were used for hybridization with Affymetrix Mouse Gene 1.0 ST array.
|
| Scan protocol |
An affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
|
| Description |
CD4+ T cells isolated from the mice after 3 dose escalation peptide immunotherapy
|
| Data processing |
Raw expression data (.CEL files) were normalized using Robust Multi-array Averaging (RMA) algorithm with the quantile normalization method. The quality control showed that an empirical intensity of 200 or less could not be reliably discriminated, and thus any values below 200 were considered as being technically undetectable.
|
| |
|
| Submission date |
Apr 12, 2013 |
| Last update date |
Jul 15, 2014 |
| Contact name |
Hai Fang |
| E-mail(s) |
hfang@cs.bris.ac.uk
|
| Organization name |
University of Bristol
|
| Department |
Department of Computer Science
|
| Street address |
Woodland Road
|
| City |
Bristol |
| ZIP/Postal code |
BS8 1UB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE46036 |
Escalating-dose peptide immunotherapy induces progressive immunoregulatory changes in self-reactive T cells |
|
