|
| Status |
Public on May 01, 2013 |
| Title |
Gene expression in BAL 48 h after allergen challenge, asthmatic1 |
| Sample type |
RNA |
| |
|
| Source name |
Gene expression in BAL 48 h after allergen challenge, asthmatic1
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Human Bronchoalveolar cells from a mild asthmatic, after allergen challenge, subject1
cell type: Bronchoalveolar cells
disease state: mild asthma
treatment: after allergen challenge
individual: subject 1
|
| Treatment protocol |
Atopic subjects underwent bronchoscopies before and 48 h after an allergen challenge. The same procedure was repeated on each donor, 1 month after an injection with mepolizumab (anti-IL-5) that decreases the amount of airway eosinophils after allergen challenge.
|
| Growth protocol |
No growth, BAL cells are used immediately after broncoscopy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from BAL cells was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
cDNA were prepared using Ambion whole transcript target labelling kit according to the manufacturer's instructions. Fragmentation and labeling of the single-stranded cDNA was performed using the Affymetrix GeneChip WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
The labeled cDNAs were hybridized to Human Gene ST 1.0 GeneChips (Affymetrix) according to the manufacturer’s protocols.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 (Affymetrix).
|
| Description |
Gene expression in BAL 48 h after allergen challenge
|
| Data processing |
Raw data (CEL files) were uploaded into ArrayStar software version 4.0 (DNASTAR Inc., Madison, WI, USA) for normalization and statistical analysis. The robust multichip analysis (RMA) algorithm was used for background correction, quantile normalization and median polish summarization.
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| |
|
| Submission date |
Apr 17, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Stephane Esnault |
| E-mail(s) |
sesnault@wisc.edu
|
| Organization name |
University of Wisconsin
|
| Department |
Medicine
|
| Street address |
600 Highland avenue
|
| City |
Madison |
| ZIP/Postal code |
53792 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE46159 |
Segmental Allergen Challenge Regulates Gene Expression In Bronchoalveolar Lavage (BAL) Cells From Atopic and Mild Asthmatic Subjects |
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