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| Status |
Public on May 01, 2013 |
| Title |
Segmental Allergen Challenge Regulates Gene Expression In Bronchoalveolar Lavage (BAL) Cells From Atopic and Mild Asthmatic Subjects |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Segmental allergen challenge increases the percentage of eosinophils in bronchoalveolar lavage (BAL) cells. Mepolizumab, an anti-IL-5 therapeutic antibody, decreases the number of eosinophils in bronchoalveolar lavages (BAL).
The use of both procedures allows to define genes that are either expressed by eosinophils or dependent on eosinophil presence in the airways.
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| Overall design |
Cells from Bronchoalveolar lavages (BAL) are obtained by bronchoscopy before (V5) and 48 h after a segmental allergen challenge (V6) in atopic and mild astmatics. This procedure was repeated in the same two subjects 2 months later and 1 month after an injection of mepolizumab (V22 is before challenge and V23 after challenge). Cells were immediately lysed and isolated total RNAs were analyzed using the Human Genome 1.0 ST GeneChip arrays (Affymetrix, Santa Clara, CA).
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| Contributor(s) |
Esnault S, Bochkov YA, Kelly EA, Jarjour NN |
| Citation(s) |
23844029 |
| |
| Submission date |
Apr 17, 2013 |
| Last update date |
Aug 23, 2019 |
| Contact name |
Stephane Esnault |
| E-mail(s) |
sesnault@wisc.edu
|
| Organization name |
University of Wisconsin
|
| Department |
Medicine
|
| Street address |
600 Highland avenue
|
| City |
Madison |
| ZIP/Postal code |
53792 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| GSM1125166 |
Gene expression in BAL before allergen challenge, asthmatic1 |
| GSM1125167 |
Gene expression in BAL 48 h after allergen challenge, asthmatic1 |
| GSM1125168 |
Gene expression in BAL before allergen challenge and 1 month after mepolizumab injection, asthmatic1 |
| GSM1125169 |
Gene expression in BAL after allergen challenge and 1 month after mepolizumab injection, asthmatic1 |
| GSM1125170 |
Gene expression in BAL before allergen challenge, asthmatic2 |
| GSM1125171 |
Gene expression in BAL 48 h after allergen challenge, asthmatic2 |
| GSM1125172 |
Gene expression in BAL before allergen challenge and 1 month after mepolizumab injection, asthmatic2 |
| GSM1125173 |
Gene expression in BAL after allergen challenge and 1 month after mepolizumab injection, asthmatic2 |
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| Relations |
| BioProject |
PRJNA197387 |
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