|
| Status |
Public on Jul 01, 2013 |
| Title |
E13.5 FL LSK HES1-GFP negative replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
age: E13.5
genotype: Hes1 GFP/WT
cell type: Lineage negative, cKit+, Sca1+ foetal liver cells from E13.5 embryo
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy Plus mini Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
|
| Label |
biotin
|
| Label protocol |
5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
|
| |
|
| Hybridization protocol |
3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
|
| Scan protocol |
Affymetrix 7G GeneArray scanner
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Genespring GX program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
May 08, 2013 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Camille Lobry |
| E-mail(s) |
camille.lobry@inserm.fr
|
| Organization name |
Inserm
|
| Department |
IRSL Inserm U944 / CNRS UMR7212
|
| Lab |
Lobry lab
|
| Street address |
16 rue de la Grange aux Belles
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE46725 |
Expression data from E13.5 Fetal Liver LSK Hes1-GFP positive and E13.5 Fetal Liver LSK Hes1-GFP negative |
| GSE46726 |
In Vivo Mapping of Notch Pathway Activity in Normal and Stress Hematopoiesis |
|
