|
| Status |
Public on Mar 04, 2016 |
| Title |
D30-2 |
| Sample type |
SRA |
| |
|
| Source name |
Adipose tissues, postnatal 30d
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Jinhua pig
tissue: subcutaneous adipose tissues
developmental stage: postnatal 30 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Subcutaneous adipose tissues were removed, flash frozen on liquid nitrogen, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Digital gene expression tag profiling (DGE). Tag length = 21 nt.
Chinese indigenous pig breed.
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to pig whole genome using Sscrofa10.2.
Genome_build: Sscrofa10.2
Supplementary_files_format_and_content: Tab-delimited text files include tag sequences, counts, and TPMs (number of transcripts per million tags).
|
| |
|
| Submission date |
May 08, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jie Zhang |
| E-mail(s) |
zhangjie813@163.com
|
| Phone |
023-46751588
|
| Organization name |
Southwest University
|
| Department |
College of Animal Science
|
| Lab |
Institute of Animal Genetics & Breeding
|
| Street address |
Xueyuan road #160
|
| City |
Rongchang |
| State/province |
Chongqing |
| ZIP/Postal code |
402460 |
| Country |
China |
| |
|
| Platform ID |
GPL11429 |
| Series (1) |
| GSE46755 |
Digital gene expression tag profiling of pig subcutaneous adipose tissue from postnatal 0 day to 7 years |
|
| Relations |
| BioSample |
SAMN02141550 |
| SRA |
SRX276208 |
