|
| Status |
Public on May 16, 2013 |
| Title |
Iron-limited 1, day 3 |
| Sample type |
RNA |
| |
|
| Source name |
Cells were grown in replete media, then washed and resuspended in iron-free media; cells were harvested on day 3
|
| Organism |
Thalassiosira pseudonana |
| Characteristics |
nutrient condition: Iron-limited
growth stage: late exponential
|
| Treatment protocol |
Exponentially growing replete cultures were collected by filtration, washed in iron-free media, and resuspended in either replete or iron-free media
|
| Growth protocol |
All cultures were maintained at 18 degree C on a 14:10 light:dark cycle with constant bubbling, growth irradiance was approximately 100 umol photons/m2/s, replete media was f/2; iron-free media was f/2 without iron added
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Amplified cDNA was fragmented and biotin-labeled with FL-Ovation cDNA Fluorescent Module (NuGEN) according to manufacturer's instructions
|
| |
|
| Hybridization protocol |
Hybridization was performed for 40 h, rotating at a speed of 10 rpm in a 65 degree C oven; Hybridized slide was washed using a commerical kit from Agilent Technologies
|
| Scan protocol |
Slide was scanned using an Agilent A2565BA microarray scanner
|
| Description |
Gene expression in iron-limited cultures in late exponential phase
|
| Data processing |
Scanned images were anlayzed and normalized signal intensities obtained by normalizng the median signal intensity to 1.
|
| |
|
| Submission date |
May 15, 2013 |
| Last update date |
May 16, 2013 |
| Contact name |
Kim Thamatrakoln |
| E-mail(s) |
thamat@marine.rutgers.edu
|
| Organization name |
Rutgers University
|
| Street address |
71 Dudley Road
|
| City |
New Brunswick |
| State/province |
New Jersey |
| ZIP/Postal code |
08901 |
| Country |
USA |
| |
|
| Platform ID |
GPL17168 |
| Series (1) |
| GSE46982 |
Whole-genome expression analysis reveals a role for death-related genes in stress acclimation of the diatom Thalasiossira pseudonana |
|
