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Sample GSM1148056 Query DataSets for GSM1148056
Status Public on Aug 26, 2013
Title siHic2
Sample type RNA
 
Source name WPMY-1 Sh2 TGFB1I1
Organism Homo sapiens
Characteristics cell line: WPMY-1
cell type: Immortalized myofibroblast
transfection: short hairpin RNA against Hic-5
treatment: 16hr vehicle
Treatment protocol Plated 2x10e4 cells/well in 6-well dishes for 48h in RPMI 1640 containing 5% hormone striped fetal bovine serum. Media was replaced with fresh hormone stripped 5% FBS with TGFB or equivalent vehicle (ethanol)
Growth protocol WPMY-1 cells were maintained in RPMI 1640 medium containing 5% fetal bovine serum.
Extracted molecule total RNA
Extraction protocol RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
Label biotin
Label protocol Biotinylated cRNA were prepared from 300ng RNA according to the standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix hybridization protocol
Scan protocol Standard Affymetrix scanning protocol
Description Immortalized myofibroblast stably transfected with short hairpin RNA against Hic-5
replicate 2
Data processing Raw data was processed in in R using Bioconductior and Limma. Array data was normalized using RMA, filtered by mapped probes and an arbitary minimum threshold.
 
Submission date May 24, 2013
Last update date Aug 26, 2013
Contact name damien leach
E-mail(s) damien.leach@adelaide.edu.au
Organization name the university of adelaide
Street address woodville road
City Adelaide
ZIP/Postal code 5011
Country Australia
 
Platform ID GPL6244
Series (1)
GSE47354 Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts

Data table header descriptions
ID_REF
VALUE Log2 Relative expression

Data table
ID_REF VALUE
7892501 5.462100716
7892502 3.782907428
7892503 3.142389263
7892504 9.383704248
7892505 3.987987892
7892506 3.644089672
7892507 4.561367341
7892508 4.4015262
7892509 11.43110191
7892510 3.307811816
7892511 2.025420204
7892512 6.876370171
7892513 3.091661848
7892514 9.918238204
7892515 6.967191773
7892516 6.8294209
7892517 5.495497322
7892518 2.217936235
7892519 4.402812462
7892520 8.561296821

Total number of rows: 31318

Table truncated, full table size 608 Kbytes.




Supplementary file Size Download File type/resource
GSM1148056_GB_E2.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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