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| Status |
Public on Aug 26, 2013 |
| Title |
Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Identifying the effect of the co-regulator Hic-5 (TGFB1I1) and TGFB on the transcriptional profile of WPMY human prostate fibroblast cells with view to further elucidating the broader biological role of Hic-5 and TGFB on fibroblast.
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| |
|
| Overall design |
Isogenic WPMY-1 fibroblasts stably transfected with short-hairpin RNA against Hic-5 or transfected with non-specific negative control short-hairpin RNA, were kept in 5% hormone stripped FBS RPMI for 48 hours and subsequently treated with either ethanol vehicle control or TGFB for 16hr. Total RNA was extracted. Five independent vehicle and five TGFB siSCR and siHic5 samples were hybrydised to Affymetrix Human Gene 1.0 ST array chips.
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| |
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| Contributor(s) |
Leach DA, Buchanan G |
| Citation(s) |
24825850 |
| |
| Submission date |
May 24, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
damien leach |
| E-mail(s) |
damien.leach@adelaide.edu.au
|
| Organization name |
the university of adelaide
|
| Street address |
woodville road
|
| City |
Adelaide |
| ZIP/Postal code |
5011 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA205278 |
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