|
| Status |
Public on Aug 26, 2013 |
| Title |
TGBscr5 |
| Sample type |
RNA |
| |
|
| Source name |
WPMY-1 SCR
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: WPMY-1
cell type: Immortalized myofibroblast
transfection: negative control short hairpin RNA
treatment: 16hr TGFB
|
| Treatment protocol |
Plated 2x10e4 cells/well in 6-well dishes for 48h in RPMI 1640 containing 5% hormone striped fetal bovine serum. Media was replaced with fresh hormone stripped 5% FBS with TGFB or equivalent vehicle (ethanol)
|
| Growth protocol |
WPMY-1 cells were maintained in RPMI 1640 medium containing 5% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 300ng RNA according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Description |
Immortalized myofibroblast stably transfected with negative control short hairpin RNA
replicate 5
|
| Data processing |
Raw data was processed in in R using Bioconductior and Limma. Array data was normalized using RMA, filtered by mapped probes and an arbitary minimum threshold.
|
| |
|
| Submission date |
May 24, 2013 |
| Last update date |
Aug 26, 2013 |
| Contact name |
damien leach |
| E-mail(s) |
damien.leach@adelaide.edu.au
|
| Organization name |
the university of adelaide
|
| Street address |
woodville road
|
| City |
Adelaide |
| ZIP/Postal code |
5011 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE47354 |
Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts |
|
