|
| Status |
Public on Jul 01, 2013 |
| Title |
head and neck squamous cell carcinoma 211 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
tumor sample
|
| Organism |
Homo sapiens |
| Characteristics |
age at operation (yrs): 56.5
gender: male
primary tumor site: Oropharyngeal
hpv infection: 0
p16 protein immunohistochemistry: 0
tnm stage: 3
tumor grade: 0
chemoradiotherapy: 0
relapse: 0
survival status: 0
smoking status: 1
alcohol drinking: 0
|
| Growth protocol |
Clinical samples
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
0.5 µg of total DNA were extracted using DNeasy and primed following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
following manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
healthy non-smoking volunteer
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
sample type: non-drinker control
tissue: peripheral blood
|
| Growth protocol |
Clinical samples
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
0.5 µg of total DNA were extracted using DNeasy and primed following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
following manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Scanned on an Agilent G4449A scanner.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| Data processing |
Agilent Feature Extraction Software (v 8.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
May 28, 2013 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Mitsuyoshi Urashima |
| E-mail(s) |
urashima@jikei.ac.jp
|
| Organization name |
Jikei University School of Medicine
|
| Street address |
Nishishimbashi 3-25-8, Minato-ku
|
| City |
Tokyo |
| ZIP/Postal code |
1058461 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10150 |
| Series (1) |
| GSE47443 |
Somatic copy number alterations associating with alcohol drinking and smoking in head and neck squamous cell carcinoma |
|
