|
| Status |
Public on Jul 01, 2013 |
| Title |
E0-E1_rep3_a |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mutant E. coli, before butanol treatment
|
| Organism |
Escherichia coli |
| Characteristics |
protocol: late log phase, LB
strain: PKH5000
|
| Treatment protocol |
1-butanol was added at a final concentration of 0.9% (vol/vol) and cells were further incubated for 30 min.
|
| Growth protocol |
Cells were grown aerobically in 50 ml LB medium until OD600 reached ~1 (late log phase).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy column
|
| Label |
Cy3
|
| Label protocol |
Labeling and hybridization were performed by the service provider (E-Biogen, Seoul, South Korea).
|
| |
|
| Channel 2 |
| Source name |
mutant E. coli, after butanol treatment
|
| Organism |
Escherichia coli |
| Characteristics |
strain: PKH5000
protocol: late log phase, LB, 30 min after butanol treatment
|
| Treatment protocol |
1-butanol was added at a final concentration of 0.9% (vol/vol) and cells were further incubated for 30 min.
|
| Growth protocol |
Cells were grown aerobically in 50 ml LB medium until OD600 reached ~1 (late log phase).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy column
|
| Label |
Cy5
|
| Label protocol |
Labeling and hybridization were performed by the service provider (E-Biogen, Seoul, South Korea).
|
| |
|
| |
| Hybridization protocol |
Labeling and hybridization were performed by the service provider (E-Biogen, Seoul, South Korea).
|
| Scan protocol |
The hybridized slides were scanned with GenePix 4000B and scanned images were analyzed by GenePix Pro 6.0.1.25.
|
| Description |
Block a, biological replicate 3 of 3 (mutant, before vs. after butanol treatment).
|
| Data processing |
Background correction and normalization (print-tip loess and quantile) were performed using the limma R package.
|
| |
|
| Submission date |
Jun 03, 2013 |
| Last update date |
Aug 03, 2015 |
| Contact name |
Haeyoung Jeong |
| E-mail(s) |
hyjeong@kribb.re.kr
|
| Phone |
+82 42 860 4237
|
| Fax |
+82 42 879 8519
|
| Organization name |
KRIBB
|
| Street address |
125 Gwahak-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL7395 |
| Series (1) |
| GSE47589 |
Transcriptome analysis of Escherichia coli C strains (wt and PKH5000, a butanol-tolerant mutant) after 1-butanol treatment |
|
