tissue: Leukocyte peripheral blood disease status: unaffected gender: male
Extracted molecule
genomic DNA
Extraction protocol
Genomic DNA was extracted from leukocyte obtained from peripheral blood using the Wizard Kit. DNA quality and quantity was assessed using both Nanodrop Spectrophotometer, PicoGreen and agarose gel electrophoresis.
Label
Biotin
Label protocol
As per manufacturer (Affymetrix)
Hybridization protocol
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol
The Arrays were then washed using Affymetrix fluidics stations (GeneChip Fluidics Station 450), and scanned using Affymetrix scanner GCS3000-7G.
