|
| Status |
Public on Jun 25, 2013 |
| Title |
Lacrimal glands at 4 weeks, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Lacrimal glands of C57BL/6.NOD-Aec1Aec2 mice at 4 weeks of age
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lacrimal glands
age: 04 week
gender: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini-Kit (Qiagen, Valencia, CA), as per the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized for 16 h at 45°C to the array chips (Affymetrix). Genechips were washed ans stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression profiles within the Lacrimal glands of C57BL/6.NOD-Aec1Aec2 mice at 4 weeks of age (representing the pre-disease stage).
|
| Data processing |
Microarray data were normalized using the “robust multi-array average” (GCRMA) algorithm.
|
| |
|
| Submission date |
Jun 20, 2013 |
| Last update date |
Jun 25, 2013 |
| Contact name |
Ammon B Peck |
| E-mail(s) |
Peck@pathology.ufl.edu
|
| Phone |
352-294-4211
|
| Organization name |
University of Florida
|
| Department |
Department of Infectious Diseases & Pathology
|
| Street address |
1945 SW 16th Avenue
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE48139 |
Differential gene expression in the Lacrimal gland during development and onset of xerostomia in Sjögren’s syndrome-like disease of the C57BL/6.NOD-Aec1Aec2 mouse. |
|
