NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1195736 Query DataSets for GSM1195736
Status Public on Jul 26, 2013
Title IL-4 (SAMPLE 9)
Sample type RNA
 
Source name monocyte-derived macrophages, IL-4
Organism Homo sapiens
Characteristics cell type: monocyte-derived macrophages
culture condition: 10% Human serum+50 ng/ml of IL-10
Treatment protocol cells were stimulated with indicated cytokines for 5 days.
Growth protocol Monocytes were isolated from buffy coats of healthy blood donors by lymphoprep density gradient centrifugation followed by plastic adherence and culture in IMDM containing 10% human pool serum, 1% penicillin/streptomycin (Invitrogen, San Diego, CA), and 0.25% ciproxin (Bayer, Leverkusen, Germany).
Extracted molecule total RNA
Extraction protocol Tripure isolation reagent ( roche) + Rneasy cleanup
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 1
Data processing Quantile normalized
 
Submission date Jul 26, 2013
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL10558
Series (2)
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
GSE49240 Similarities and differences between human activated macrophages I

Data table header descriptions
ID_REF
VALUE RMA signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 5.838428988 0.72857
ILMN_2055271 6.036293924 0.97532
ILMN_1736007 5.874888226 0.81429
ILMN_2383229 5.730833249 0.43377
ILMN_1806310 5.788015599 0.6039
ILMN_1779670 5.777701179 0.58052
ILMN_1653355 5.863386933 0.78571
ILMN_1717783 5.572202497 0.12727
ILMN_1705025 6.023843109 0.97532
ILMN_1814316 5.707456873 0.36883
ILMN_2359168 5.562252594 0.11039
ILMN_1731507 5.6934039 0.34416
ILMN_1787689 5.870381161 0.8026
ILMN_3241953 6.756292906 1
ILMN_1745607 9.256490119 1
ILMN_2136495 5.572202497 0.12597
ILMN_1668111 5.85858772 0.77662
ILMN_2295559 5.631709065 0.22078
ILMN_1735045 6.146232561 0.9961
ILMN_1680754 5.863386933 0.78701

Total number of rows: 47311

Table truncated, full table size 1439 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap