|
| Status |
Public on Jul 26, 2013 |
| Title |
Similarities and differences between human activated macrophages I |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The human AA dataset 5 includes the gene expression profile of monocyte-derived macrophages isolated from blood donor buffy coats and plated on TCP in medium containing 10% autologous serum. Cells were stimulated for 120 hours with 50 ng/ml of IL-4, 50 U/ml of IFN-γ plus 12.5 ng/ml of TNF-α, 50 ng/ml of M-CSF or 50 ng/ml of IL-10. RNA samples were isolated using TriPure isolation reagent (Roche) and the RNeasy Mini Kit-Qiagen Clean up method. The transcriptional profile was evaluated in two independent cell preparations, each derived from a different single donor using the HT12 V4 R2 bead chip arrays from Illumina.
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| |
|
| Overall design |
Total RNA obtained from monocyte-derived macrophages exposed to representative polarizing cytokines
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| |
|
| Contributor(s) |
Cobos Jimenez V, Martinez FO, Gordon S, Hamann J, Kookstra N |
| Citation(s) |
23293084 |
| |
| Submission date |
Jul 26, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
|
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| This SubSeries is part of SuperSeries: |
| GSE35495 |
Genome-wide analysis of human and mouse macrophages, resting and activated |
|
| Relations |
| BioProject |
PRJNA213374 |
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