|
| Status |
Public on Oct 16, 2006 |
| Title |
Calicheamicin, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast treated with Calicheamicin
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Yeast strain DBY747 treated with calicheamicin
Yeast treated with calicheamicin
|
| Treatment protocol |
Yeast strain DBY747 was treated with calicheamicin for 15min resulting in 20-25% lethality
|
| Growth protocol |
yeast were grown in YPD to mid-log phase and treated with agent
|
| Extracted molecule |
total RNA |
| Extraction protocol |
hot phenol extraction of total RNA was performed
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on GeneChip Yeast S98.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from yeast treated with calicheamicin
|
| Data processing |
The data were analyzed with Robust multichip analysis in RMA express
|
| |
|
| Submission date |
Jul 13, 2006 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
rfry@unc.edu
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE5301 |
Expression data from yeast treated with enediynes compared to gamma radiation |
|
