|
| Status |
Public on Feb 10, 2014 |
| Title |
human neural progenitor cell line_Control_replicate4 |
| Sample type |
RNA |
| |
|
| Source name |
control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: CTXOE03 cell line
gender: Male
cell type: Neural progenitor
brain region of origin: Fetal cortical neuroepithelium
|
| Treatment protocol |
Transfection of either control, mirVanaTM miRNA inhibitor (MH10513; Life Technologies) or a mirVanaTM miRNA mimic of the miR-137 precursor (MC10513; Life Technologies). Cells were harvested using acutase 3 days after transfection.
|
| Growth protocol |
Cells were maintained as described by Pollock K et al, Exp Neurol. 2006 May;199(1):143-55.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Trizol extraction, RNA dissolved in water.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared using the Custom Illumina® TotalPrep™-96 Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scan protocol
|
| Description |
Con_4
|
| Data processing |
Sample probe profile were exported from GenomeStudio. Data were then imported into lumi (bioconductor) and Variance Stabilizing transformation applied, Robust Spline Normalized and log2 transformed.
|
| |
|
| Submission date |
Aug 12, 2013 |
| Last update date |
Feb 10, 2014 |
| Contact name |
Matthew James Hill |
| Organization name |
Cardiff University
|
| Street address |
Maindy Road
|
| City |
Cardiff |
| ZIP/Postal code |
CF24 4HQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE49793 |
Transcriptional consequences of manipulating the candidate schizophrenia susceptibility gene miR-137 in human neural progenitor cells |
|
