|
| Status |
Public on Jan 17, 2014 |
| Title |
HumanDC RNA Sample8 Non-infected 18hours rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
infection status: Non-infected
|
| Treatment protocol |
We infected DCs with an MTB strain expressing green-fluorescent protein (H37Rv)
|
| Growth protocol |
Blood monocytes were purified from PBMCs by positive selection with magnetic CD14 MicroBeads. In order to derive dendritic cells, monocytes were cultured for 5 days in RPMI 1640 supplemented with 10% heat-inactivated FCS , L-glutamina, GM-CSF (20 ng/ml; Immunotools), and IL-4 (20 ng/ml; Immunotools). Cell cultures were fed every 2 days with complete medium supplemented with the cytokines previously mentioned.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the non-infected DCs and the MTB-infected DCs using the miRNeasy kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
As recommended by Agilent.
|
| |
|
| Hybridization protocol |
As recommended by Agilent.
|
| Scan protocol |
As recommended by Agilent.
|
| Description |
microRNA expression after 18 hours Non-infected DCs
|
| Data processing |
Bioconductor package AgiMicrorna.
|
| |
|
| Submission date |
Aug 18, 2013 |
| Last update date |
Jan 17, 2014 |
| Contact name |
Katherine Siddle |
| Organization name |
Institut Pasteur
|
| Street address |
28 rue du Dr Roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL16770 |
| Series (1) |
| GSE49951 |
A Genomic Portrait of the Genetic Architecture and Regulatory Impact of microRNA Expression in Response to Infection |
|
