|
Status |
Public on Jan 17, 2014 |
Title |
HumanDC RNA Sample51 Non-infected 18hours rep1 |
Sample type |
RNA |
|
|
Source name |
Human dendritic cells
|
Organism |
Homo sapiens |
Characteristics |
gender: Male infection status: Non-infected
|
Treatment protocol |
We infected DCs with an MTB strain expressing green-fluorescent protein (H37Rv)
|
Growth protocol |
Blood monocytes were purified from PBMCs by positive selection with magnetic CD14 MicroBeads. In order to derive dendritic cells, monocytes were cultured for 5 days in RPMI 1640 supplemented with 10% heat-inactivated FCS , L-glutamina, GM-CSF (20 ng/ml; Immunotools), and IL-4 (20 ng/ml; Immunotools). Cell cultures were fed every 2 days with complete medium supplemented with the cytokines previously mentioned.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the non-infected DCs and the MTB-infected DCs using the miRNeasy kit (Qiagen).
|
Label |
Cy3
|
Label protocol |
As recommended by Agilent.
|
|
|
Hybridization protocol |
As recommended by Agilent.
|
Scan protocol |
As recommended by Agilent.
|
Description |
microRNA expression after 18 hours Non-infected DCs
|
Data processing |
Bioconductor package AgiMicrorna.
|
|
|
Submission date |
Aug 18, 2013 |
Last update date |
Jan 17, 2014 |
Contact name |
Katherine Siddle |
Organization name |
Institut Pasteur
|
Street address |
28 rue du Dr Roux
|
City |
Paris |
ZIP/Postal code |
75015 |
Country |
France |
|
|
Platform ID |
GPL16770 |
Series (1) |
GSE49951 |
A Genomic Portrait of the Genetic Architecture and Regulatory Impact of microRNA Expression in Response to Infection |
|