|
| Status |
Public on Aug 24, 2013 |
| Title |
Station GD 123 m Baltic #5A |
| Sample type |
mixed |
| |
|
| Channel 1 |
| Source name |
70-mer probe
|
| Organism |
artificial sequences |
| Characteristics |
sample type: Universal Internal Standard + 70-mer
std. molecule: 20-mer DNA oligo
description: 70-mer probe
|
| Extracted molecule |
other |
| Extraction protocol |
DNA was extracted from filtered samples using the Qiagen Allprep kit
|
| Label |
Cy5
|
| Label protocol |
Targets were linearly ampified using Klenow and the dNTP mix included dUaa. The amplified product was post labeled by conjugation of Cy3 to the dUaa
|
| |
|
| Channel 2 |
| Source name |
estuary seawater
|
| Organism |
aquatic metagenome |
| Characteristics |
location, date: Stn.15 03.7.201.
location: 57 19.3315N, 020 03.0142E
molecule: random priming amplified DNA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from filtered samples using the Qiagen Allprep kit
|
| Label |
Cy3
|
| Label protocol |
Targets were linearly ampified using Klenow and the dNTP mix included dUaa. The amplified product was post labeled by conjugation of Cy3 to the dUaa
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized to single or duplicate arrays by overnight incubation at 64°C and washed.
|
| Scan protocol |
The arrays were scanned with a laser scanner (Molecular Devices 4200)
Images were analyzed with Gene Pix Pro 6.0 software (Molecular Devices)
|
| Description |
1000 ng random priming amplified DNA from Baltic seawater
|
| Data processing |
Quality control was employed to remove noise by eliminating features in which the Cy3 signal was not at least twice the value of the control probes
|
| |
|
| Submission date |
Aug 23, 2013 |
| Last update date |
Aug 24, 2013 |
| Contact name |
Bess B Ward |
| E-mail(s) |
bbw@princeton.edu
|
| Phone |
6092585150
|
| Organization name |
Princeton University
|
| Department |
Geosciences
|
| Lab |
Guyot Hall
|
| Street address |
Washington Road
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
| |
|
| Platform ID |
GPL17620 |
| Series (1) |
| GSE50164 |
Hydrodynamic conditions shape ammonia-oxidizer communities in the central Baltic Sea water column |
|
