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Platform GPL17620 Query DataSets for GPL17620
Status Public on Aug 24, 2013
Title BC012
Technology type spotted oligonucleotide
Distribution non-commercial
Organism artificial sequences
Manufacturer Princeton Microarray Facility
Manufacture protocol 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis.
2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50% DMSO and were spotted on CMT-GAPS amino silane-coated glass slides (Corning, Corning, NY).
3. After printing, the slides were baked at 80°C for 3 h and stored in the dark at room temperature under slight vacuum in a dessicator.
 
 
Submission date Aug 23, 2013
Last update date Aug 24, 2013
Contact name Bess B Ward
E-mail(s) bbw@princeton.edu
Phone 6092585150
Organization name Princeton University
Department Geosciences
Lab Guyot Hall
Street address Washington Road
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
 
Samples (20) GSM1214975, GSM1214976, GSM1214977, GSM1214978, GSM1214994, GSM1214995 
Series (2)
GSE50163 Bacteria are the dominant ammonia oxidizers in coastal sediments of the Western English Channel
GSE50164 Hydrodynamic conditions shape ammonia-oxidizer communities in the central Baltic Sea water column

Data table header descriptions
ID
GB_ACC GenBank accession number of sequence used to design oligonucleotide probe
GENE_SYMBOL
SEQUENCE

Data table
ID GB_ACC GENE_SYMBOL SEQUENCE
AOA1 AB373281 AOA amoA GGTATTCTCAATATCGCAAACGTTGATGCTTATTGTAGGTGCAACATATTACCTAACATTTACAGGCGTT
AOA2 EF414277 AOA amoA AGTATTCTCAATATCTCAAACATTAATGCTCACTGTAGGTGCATGTTACTATCTCACATTCACTGGAGTT
AOA3 AM260489 AOA amoA CATATTCTCAATATCGCAAACACTGATGTTGGTAGTCGGCGCATGCTACTACCTGACATTCACAGGAGTT
AOA4 DQ501003 AOA amoA AGTGTTCTCCATCTCGCAATCTACCATGCTTGCGGTGGGCGCGATATACTACATGCTCTTCACAGGAGTA
AOA5 DQ501119 AOA amoA AGTGTTCTCCATTGGAAACATTCTCATGATTTCTGTTGGTGCAACATACTATCTGTTATTTACAGGAGTT
AOA6 EF207214 AOA amoA AGTATTTTCGATCTCACAATCCACTATGCTTGCAGTAGGAGCGATATACTATATGCTCTTCACAGGAGTT
AOA7 EU339381 AOA amoA AGTGTTCTCAATATCCCAATCAACCATGCTTGTAGTTGGTGCAATCTATTACATGCTATTCACCGGAGTA
AOA8 EF414247 AOA amoA AGTATTTTCAATATCACAAACTTTGATGCTTGTGGTAGGTGCATGCTACTATCTGACATTTACAGGTGTT
AOA9 EU340536 AOA amoA AGTATTTTCCATTGCCAACACTTTGATGACTGTTGTTGGTGCTGTGTATTATCTTACATTTACTGGTGTG
AOA10 EU339454 AOA amoA GGTATTTTCAGTATCACAGACACTCATGCTTGCTGTCGGTGCCGCGTACTACCTTACATTCACTGGAGTT
AOA11 EU339389 AOA amoA GATATTCACGTTATCACAATCACTGATGCTCGCAGTTGGTGCAGCATATTACCTAACGTTCACTGGTGTT
AOA12 DQ534884 AOA amoA CGTGTTCTCTATATCGCAGTCAACGATGCTTGTTGTGGGTGCCATCTATTACATGTTGTTTACGGGAGTG
AOA13 EF382483 AOA amoA AGTATTTTCAATAGGAAATATTCTAATGACAGCTGTAGGTGCTACGTATTACCTAACATTTACAGGCGTT
AOA14 JF969803.1 AOA amoA AGTATTTTCCATCTCACAAACTTTGATGCTTTGTGTTGGTGCGACATATTACCTAACATTTACAGGAGTT
AOA15 EU025184 AOA amoA AGTATTCTCAATCTCACAAACTTTGATGTTAACTGTGGGTGCAGTATACTATCTTACATTTACCGGTGTT
AOA16 EU022770 AOA amoA TGTCTTTTCAATATCACAAACACTGATGTTAGTAGTCGGTGCTACGTATTATTTGACATTCACAGGTGTT
AOA17 EU553403 AOA amoA GGTATTCTCGATCTCGCAGTCGACTATGCTCGCTGTGGGTGCGATACACTACATGTTGTTCACAGGCGTT
AOA18 DQ501021 AOA amoA AGTATTTTCAATTGGAAACATTTTGATGGTTTCAGTTGGTGCTACATATTACCTCTTATTCACAGGGGTT
AOA19 EU553389 AOA amoA AGTATTTTCAATTTCGCAATCTACGATGTTGGTTGTGGGAGCAATATACTACATGCTCTTTACTGGAGTC
AOA20 EF382430 AOA amoA GGTATTCTCTATATCGCAAACTTCTATGCTTACTGTTGGTGCAGTATATTATCTGACATTTACAGGAGTT

Total number of rows: 31

Table truncated, full table size 2 Kbytes.




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