|
Status |
Public on Aug 24, 2013 |
Title |
Station GD 123 m Baltic #6A |
Sample type |
mixed |
|
|
Channel 1 |
Source name |
70-mer probe
|
Organism |
artificial sequences |
Characteristics |
sample type: Universal Internal Standard + 70-mer std. molecule: 20-mer DNA oligo description: 70-mer probe
|
Extracted molecule |
other |
Extraction protocol |
DNA was extracted from filtered samples using the Qiagen Allprep kit
|
Label |
Cy5
|
Label protocol |
Targets were linearly ampified using Klenow and the dNTP mix included dUaa. The amplified product was post labeled by conjugation of Cy3 to the dUaa
|
|
|
Channel 2 |
Source name |
estuary seawater
|
Organism |
aquatic metagenome |
Characteristics |
location, date: Stn.15 03.7.201. location: 57 19.3315N, 020 03.0142E molecule: random priming amplified DNA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from filtered samples using the Qiagen Allprep kit
|
Label |
Cy3
|
Label protocol |
Targets were linearly ampified using Klenow and the dNTP mix included dUaa. The amplified product was post labeled by conjugation of Cy3 to the dUaa
|
|
|
|
Hybridization protocol |
Samples were hybridized to single or duplicate arrays by overnight incubation at 64°C and washed.
|
Scan protocol |
The arrays were scanned with a laser scanner (Molecular Devices 4200) Images were analyzed with Gene Pix Pro 6.0 software (Molecular Devices)
|
Description |
1000 ng random priming amplified DNA from Baltic seawater
|
Data processing |
Quality control was employed to remove noise by eliminating features in which the Cy3 signal was not at least twice the value of the control probes
|
|
|
Submission date |
Aug 23, 2013 |
Last update date |
Aug 24, 2013 |
Contact name |
Bess B Ward |
E-mail(s) |
bbw@princeton.edu
|
Phone |
6092585150
|
Organization name |
Princeton University
|
Department |
Geosciences
|
Lab |
Guyot Hall
|
Street address |
Washington Road
|
City |
Princeton |
State/province |
NJ |
ZIP/Postal code |
08544 |
Country |
USA |
|
|
Platform ID |
GPL17620 |
Series (1) |
GSE50164 |
Hydrodynamic conditions shape ammonia-oxidizer communities in the central Baltic Sea water column |
|